pathwayPROFILER

A Comprehensive Assay for Analysis of Pathway Activities

The pathwayPROFILER assay is designed to measure the activities of various disease-relevant cellular pathways, including:

MAP kinase signaling
Calcium signaling
cAMP signaling
Immune signalling
Metabolic signaling
Synaptic activity

Our innovative approach encompasses a diverse range of biological targets and offers in-depth signaling pathway measurements, all assessed in parallel. This unique capability allows for the simultaneous detection of drug effects on disease-relevant pathway activities that can be altered in a disease state, providing a comprehensive analysis that significantly enhances the understanding of polypharmacology.

Methodology

The pathwayPROFILER assay employs genetically engineered biosensors coupled with unique molecular barcodes. These biosensors are integrated into living cells, where they function as reporters for signaling pathway dynamics. Upon treatment with drugs or other stimuli, changes in the activity of these targets and pathways are recorded. The molecular barcodes then allow for the precise identification and quantification of these changes using advanced sequencing methods.

Key Features

Single Measurement: pathwayPROFILER achieves high complexity in a single measurement by integrating numerous individual assays. Each assay measures a defined pathway’s activity within cells.
Proprietary Barcoding Technology: Utilizing our unique proprietary barcoding technology, pathwayPROFILER assay leverages molecular barcodes—short nucleotide sequences (DNA or RNA). These barcodes enable the unambiguous identification of cellular samples and serve as reporter molecules, indicating the activity of signaling pathways.
Highly Parallel Analysis: Our barcoding technology facilitates a highly parallel analysis within a single experiment, allowing for the simultaneous measurement of multiple targets and pathways.
Next-Generation Sequencing: The activity of targets and physiological signaling pathways is quantified through next-generation sequencing technologies, ensuring precise and comprehensive data collection.

Applications

Drug Discovery and Development: Assess the impact of new compounds on multiple disease-relevant targets and pathways simultaneously, enhancing the understanding of their polypharmacological effects including efficacy, potency & side effects.
Pathway Analysis: nvestigate the complex interactions within signaling networks in living cells, providing insights into cellular responses to compound treatments.
Personalized Medicine: Tailor therapeutic strategies based on comprehensive pathway activity profiles, improving treatment efficacy and patient outcomes.

Collaboration is part of our DNA!

Explore the capabilities of pathwayPROFILER to advance your research and development projects. For more information about pathwayPROFILER and how it can benefit your research, please contact our team of experts.

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